RFC4 Antikörper

Produktnummer ABIN190320

Dieses Anti-RFC4-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von RFC4 in WB und IF/ICC. Geeignet für Human.

Kurzübersicht für RFC4 Antikörper (ABIN190320)

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RFC4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Klon: 1320

Produktdetails anti-RFC4 Antikörper

Verwendungszweck: Anti-Replication Factor C p37 Mouse Monoclonal Antibody

Spezifität: This antibody recognizes the p37 subunit of human and yeast (S. cerevisiae) Replication Factor C (RFC). RFC is a heteropentameric protein consisting of p140, p40, p38, p37, and p36 subunits, RFC participates in targeting DNA polymerase delta processivity factor (PCNA) on to a primed DNA template during DNA synthesis and repair. RFC has also been associated with DNA repair in conjunction with a PCNA-like heterotrimer of Hus1, Rad1, and Rad9.

Kreuzreaktivität: Human, Saccharomyces cerevisiae

Immunogen: Recombinant protein corresponding to the p37 subunit of human Replication Factor C expressed in E. coli.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at 1:2,000 dilution. A band of 37 kD is detected.
Positive controls: HeLa cell lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Konzentration: Lot specific

Buffer: PBS, pH 7.4

Lagerung: -80 °C

Informationen zur Lagerung: This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.

Details zu RFC4

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Andere Bezeichnung: RFC4 / RFC37

Hintergrund: Replication factor C subunit 2,The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP (By similarity). {ECO:0000250}.,Nucleus,Activator 1 40 kDa subunit, A1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40

UniProt: P35250

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA