This antibody recognizes human DNA Ligase I (125 kD), one of the four ligase proteins found in mammalian cells that are required for the joining of DNA strand breaks that occur during DNA replication, repair, and recombination.
Kreuzreaktivität
Human
Immunogen
Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells.
LIG1
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Immunoblotting: use at 1-5 μg/mL. Immunoprecipitation: use at 1-5 μg/mL. Positive controls: MCF-7 cells or recombinant protein.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Dilute in PBS or medium which is identical to that used in the assay system.
Konzentration
Lot specific
Buffer
PBS, pH 7.4
Lagerung
-80 °C
Informationen zur Lagerung
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Target
LIG1
(Ligase I, DNA, ATP-Dependent (LIG1))
Andere Bezeichnung
DNA ligase 1
Hintergrund
DNA ligase 1,DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.,Nucleus.,EC 6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1