COPS5 Antikörper (AA 1-334)

Produktnummer ABIN190283

Der Maus Monoklonal Anti-COPS5-Antikörper wurde für WB und IP validiert. Er ist geeignet, COPS5 in Proben von Maus zu detektieren.

Kurzübersicht für COPS5 Antikörper (AA 1-334) (ABIN190283)

Target: COPS5 (COP9 Constitutive Photomorphogenic Homolog Subunit 5 (Arabidopsis) (COPS5))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser COPS5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 8H8-5

Produktdetails anti-COPS5 Antikörper

Bindungsspezifität: AA 1-334

Verwendungszweck: Anti-JAB-1 Mouse Monoclonal Antibody

Spezifität: This antibody recognizes human and mouse JAB-1.

Kreuzreaktivität: Human, Maus

Immunogen: Mouse JAB-1 gst-fusion protein (aa 1-334) expressed in E. coli.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoblotting and
Immunoprecipitation: use at 0.5-1 μg/mL.
Positive controls: 293 cells and recombinant fusion protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Konzentration: Lot specific

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Details zu COPS5

Target: COPS5 (COP9 Constitutive Photomorphogenic Homolog Subunit 5 (Arabidopsis) (COPS5))

Andere Bezeichnung: COPS5

Hintergrund: COP9 signalosome complex subunit 5,Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. {PubMed:11285227, PubMed:11337588, PubMed:12628923, PubMed:12732143, PubMed:19214193, PubMed:20978819, PubMed:22609399, PubMed:9535219}.,JAB-1 is a 38 kD protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the â€signalosome†which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants which plays an important role in light-mediated plant development. Although the specific function of JAB-1 is unclear, it appears to be involved in targeting proteins for degradation by the proteasome.,Cytoplasm, cytosol, Nucleus, Cytoplasm, perinuclear region, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle,SGN5, Signalosome subunit 5, EC 3.4.-.-, Jun activation domain-binding protein 1

UniProt: Q92905

Pathways: Zellzyklus