Calreticulin Antikörper (CALR)

Details for Product anti-CALR Antibody No. ABIN6567010
Target
Reaktivität
Human, Maus, Ratte
183
121
99
42
38
37
36
36
34
33
28
28
7
6
3
2
1
1
1
1
Wirt
Kaninchen
151
25
12
3
1
Klonalität
Polyklonal
Konjugat
Dieser Calreticulin Antikörper ist unkonjugiert
14
9
8
7
5
5
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
157
92
72
54
44
41
40
29
13
9
7
4
3
1
1
1
1
1
1
Optionen
Immunogen Recombinant protein of human CALR
Isotyp IgG
Reinigung Affinity purification
Target
Andere Bezeichnung CALR (CALR Antibody Abstract)
Hintergrund

Synonyms: Autoantigen RO,CALR,CALR protein,CALR,Calregulin,Calreticulin,cC1qR,CRP55,CRT,CRTC,Endoplasmic reticulum resident protein 60,Epididymis secretory sperm binding protein Li 99n,ERp60,FLJ26680,grp60,HACBP,HEL S 99n,RO,Sicca syndrome antigen A (autoantigen Ro,calreticulin),Sicca syndrome antigen A,SSA

Background: Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Molekulargewicht

Observed_MW: 55kDa

Calculated_MW: 48kDa

Gen-ID 811
UniProt P27797
Pathways Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded protein response
Applikations-hinweise WB 1:500 - 1:2000 IF 1:20 - 1:100 IP 1:50 - 1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C. Avoid freeze / thaw cycles.
Bilder
Image no. 1 for anti-Calreticulin (CALR) antibody (ABIN6567010) Western blot analysis of extracts of various cell lines, using CALR antibody.
Image no. 2 for anti-Calreticulin (CALR) antibody (ABIN6567010) Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug CALR antibody.
Image no. 3 for anti-Calreticulin (CALR) antibody (ABIN6567010) Immunofluorescence analysis of A549 cells using CALR antibody.