DFFA Antikörper

Produktnummer ABIN6566873

Der Kaninchen Polyklonal Anti-DFFA-Antikörper wurde für WB und IF validiert. Er ist geeignet, DFFA in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für DFFA Antikörper (ABIN6566873)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DFFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-DFFA Antikörper

Aufreinigung: Affinity purification

Immunogen: Recombinant protein of human DFFA

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000 IF 1:50 - 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Andere Bezeichnung: DFFA

Hintergrund:

Synonyms: A330085O09Rik,Caspase activated deoxyribonuclease inhibitor short form,DFF 1,DFF 45,DFF alpha,DFF-45,DFF1,DFF35,DFF45,DFFA,Dffa DNA fragmentation factor,alpha subunit,DFFA,DNA fragmentation factor 45 kDa subunit,DNA Fragmentation Factor Alpha Subunit,DNA fragmentation factor subunit alpha,DNA fragmentation factor,45 kD,alpha subunit,DNA fragmentation factor,45  kDa,alpha polypeptide (DFFA),transcript variant 1,DNA fragmentation factor,45  kDa,alpha polypeptide,DNA fragmentation factor,alpha subunit,DNAation factor 45 kDa subunit,H13,ICAD,ICAD L,ICAD S,Inhibitor of CAD,Inhibitor of Caspase Activated DNase,MGC143066,OTTHUMP00000001903,OTTHUMP00000001904,RP23 121D17.3

Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molekulargewicht:

Observed_MW: 35kDa, 45kDa

Calculated_MW: 29kDa/36kDa

Gen-ID: 1676

UniProt: O00273

Pathways: Apoptose, Caspase Kaskade in der Apoptose