Golgi Antikörper

Produktnummer ABIN6297710

Der Maus Monoklonal Anti--Antikörper wurde für FACS, IF, ICC, WB und IHC validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Golgi Antikörper (ABIN6297710)

Target: Golgi

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails

Verwendungszweck: Mouse anti-Human Golgi Antibody [Sodium Azide Free]

Spezifität: Golgi complex in cytoplasm

Aufreinigung: SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.

Immunogen: SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (0.5-1 μg/million cells in 0.1ml)
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Antigendetails

Target: Golgi

Hintergrund:

Target Description: This mAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.