Golgi Antikörper

Produktnummer ABIN6251934

Produktdetails

Target: Golgi

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blotting (WB), Immunohistochemistry (IHC)

Aufreinigung: Protein G affinity chromatography

Immunogen: SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.

Klon: SPM581

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (0.5-1 μg/million cells in 0.1ml)

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.5-1 μg/mL

ICC (Acetone or paraformaldehyde fixed): 0.5-1 μg/mL for 30 min

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

The optimal dilution of the anti-Golgi antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Antigendetails

Target: Golgi

Hintergrund: This mAb recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.