ICAM-3/CD50 Antikörper

Produktnummer ABIN6297692

Der Maus Monoklonal Anti-ICAM-3/CD50-Antikörper wurde für FACS, IF und IHC validiert. Er ist geeignet, ICAM-3/CD50 in Proben von Human zu detektieren.

Kurzübersicht für ICAM-3/CD50 Antikörper (ABIN6297692)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICAM-3/CD50 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails anti-ICAM-3/CD50 Antikörper

Verwendungszweck: Mouse anti-Human ICAM-3 Antibody [Sodium Azide Free]

Spezifität: Cell surface

Aufreinigung: Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.

Immunogen: Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Andere Bezeichnung: Intercellular adhesion molecule 3

Hintergrund:

Target Description: Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This mAb is excellent for staining of formalin/paraffin tissues.

Gene Symbol: ICAM3

Gen-ID: 3385

UniProt: P32942