CD53 Antikörper

Produktnummer ABIN6297690

Dieses Anti-CD53-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD53 in FACS, IF und Func. Geeignet für Human.

Kurzübersicht für CD53 Antikörper (ABIN6297690)

Target: CD53

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD53 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Produktdetails anti-CD53 Antikörper

Verwendungszweck: Mouse anti-Human CD53 Antibody [Sodium Azide Free]

Spezifität: Cell surface

Aufreinigung: Stimulated human leukocytes were used as the immunogen for the CD53 antibody.

Immunogen: Stimulated human leukocytes were used as the immunogen for the CD53 antibody.

Anwendungsinformationen

Applikationshinweise: Functional Activity (Order SAF formulation)
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu CD53

Target: CD53

Andere Bezeichnung: Leukocyte surface antigen CD53

Hintergrund:

Target Description: Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI, Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This mAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

Gene Symbol: CD53///MOX44///TSPAN25

Gen-ID: 963

UniProt: P19397