CD43 Antikörper

Produktnummer ABIN6297682

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD43 in FACS, WB, IHC und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD43 Antikörper (ABIN6297682)

Target: CD43 (SPN) (Sialophorin (SPN))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-CD43 Antikörper

Verwendungszweck: Mouse anti-Human CD43 Antibody [Sodium Azide Free]

Spezifität: Cell surface

Aufreinigung: Stimulated human leukocytes were used as the immunogen for the CD43 antibody.

Immunogen: Stimulated human leukocytes were used as the immunogen for the CD43 antibody.

Anwendungsinformationen

Applikationshinweise: Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: Leukosialin

Hintergrund:

Target Description: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Gene Symbol: SPN///CD43

Gen-ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity