Nucleolin Antikörper

Produktnummer ABIN6297426

Dieses Anti-Nucleolin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Nucleolin in WB, IHC, IF und FACS. Geeignet für Human, Maus, Ratte, Rind (Kuh), Schwein, Kaninchen, Meerschweinchen, Huhn, Cat und Wüstenrennmaus.

Kurzübersicht für Nucleolin Antikörper (ABIN6297426)

Target: Nucleolin (NCL)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Kaninchen, Meerschweinchen, Huhn, Cat, Wüstenrennmaus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nucleolin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-Nucleolin Antikörper

Verwendungszweck: Mouse anti-Human/Mouse/Rat/Guinea Pig/Gerbil/Cat/Pig/Rabbit/Cow/Chicken Nucleolin Antibody [Sodium Azide Free]

Spezifität: Nucleoli

Aufreinigung: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Immunogen: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
IF: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Western blot: 0.5-1 μg/mL
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Nucleolin

Target: Nucleolin (NCL)

Andere Bezeichnung: Nucleolin

Hintergrund:

Target Description: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Gene Symbol: NCL

Gen-ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization