Cytochrome C Antikörper

Produktnummer ABIN6296905

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cytochrome C in WB, FACS, IF und IP. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für Cytochrome C Antikörper (ABIN6296905)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytochrome C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-Cytochrome C Antikörper

Verwendungszweck: Mouse anti-Human/Mouse/Rat Cytochrome C Antibody [Sodium Azide Free]

Spezifität: Cytoplasmic

Aufreinigung: Rat full-length protein was used as the immunogen for the Cytochrome C antibody.

Immunogen: Rat full-length protein was used as the immunogen for the Cytochrome C antibody.

Anwendungsinformationen

Applikationshinweise: Western blot: 1-2 μg/mL
Immunoprecipitation: 2 μg/mg of lysate
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: Cytochrome c

Hintergrund:

Target Description: Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Gene Symbol: CYCS///CYC

Gen-ID: 54205

UniProt: P99999

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity