S100A9 Antikörper

Produktnummer ABIN6296494

Dieses Maus Monoklonal-Antikörper erkennt spezifisch S100A9 in WB, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für S100A9 Antikörper (ABIN6296494)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-S100A9 Antikörper

Verwendungszweck: Mouse anti-Human/Rat S100A9 Antibody [Sodium Azide Free]

Spezifität: Cytoplasmic, extracellular (secreted) and cell surface

Aufreinigung: Human peripheral blood monocyte components were used as the immunogen for the S100A9 antibody.

Immunogen: Human peripheral blood monocyte components were used as the immunogen for the S100A9 antibody.

Anwendungsinformationen

Applikationshinweise: Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Andere Bezeichnung: Protein S100-A9

Hintergrund:

Target Description: This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer's cells of the liver and Langerhan's cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Gene Symbol: S100A9///CAGB///CFAG///MRP14

Gen-ID: 6280

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine