Desmoglein 2 Antikörper

Produktnummer ABIN6296077

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Desmoglein 2 in WB, IF, FACS und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Desmoglein 2 Antikörper (ABIN6296077)

Target: Desmoglein 2 (DSG2)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Produktdetails anti-Desmoglein 2 Antikörper

Verwendungszweck: Mouse anti-Human Desmoglein 2 Antibody / DSG2 [Sodium Azide Free]

Spezifität: Cell surface

Aufreinigung: A recombinant human DSG2 protein was used as the immunogen for the Desmoglein 2 antibody.

Immunogen: A recombinant human DSG2 protein was used as the immunogen for the Desmoglein 2 antibody.

Anwendungsinformationen

Applikationshinweise: FACS: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
WB: 1-2 μg/mL
Immunoprecipitation: suitable

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Desmoglein 2

Target: Desmoglein 2 (DSG2)

Andere Bezeichnung: Desmoglein-2

Hintergrund:

Target Description: Recognizes a protein of 165 kDa, identified as Desmoglein-2 (DSG2). This monoclonal antibody recognizes the extracellular domain of human desmoglein-2. Desmoglein-2 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).

Gene Symbol: DSG2///CDHF5

Gen-ID: 1829

UniProt: Q14126

Pathways: Cell-Cell Junction Organization