N-Cadherin Antikörper (Extracellular Domain)

Produktnummer ABIN6295448

Der Maus Monoklonal Anti-N-Cadherin-Antikörper wurde für WB, IHC, IF und FACS validiert. Er ist geeignet, N-Cadherin in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für N-Cadherin Antikörper (Extracellular Domain) (ABIN6295448)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser N-Cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-N-Cadherin Antikörper

Bindungsspezifität: Extracellular Domain

Verwendungszweck: Mouse anti-Human/Mouse/Rat N-Cadherin Antibody / CDH2 [Sodium Azide Free]

Spezifität: Cell surface, cytoplasmic

Aufreinigung: A human partial recombinant protein corresponding to the N-Cadherin extracellular domain was used as the immunogen for this CDH2 antibody.

Immunogen: A human partial recombinant protein corresponding to the N-Cadherin extracellular domain was used as the immunogen for this CDH2 antibody.

Anwendungsinformationen

Applikationshinweise: FACS: 0.5-1 μg/10^6 cells in 0.1ml
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: Cadherin-2

Hintergrund:

Target Description: Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CDH2 and CD325. CDH2 is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CDH2 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, the protein has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, it is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Gene Symbol: CDH2///CDHN///NCAD

Gen-ID: 1000

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane