CD3 epsilon Antikörper (Cytoplasmic Domain)

Produktnummer ABIN6294325

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD3 epsilon in FACS, WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Schwein, Rind (Kuh), Pferd, Kaninchen und Meerschweinchen.

Kurzübersicht für CD3 epsilon Antikörper (Cytoplasmic Domain) (ABIN6294325)

Target: CD3 epsilon (CD3E)

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-CD3 epsilon Antikörper

Bindungsspezifität: AA 156-168, Cytoplasmic Domain

Verwendungszweck: Mouse anti-Human/Mouse/Rat/Rabbit/Guinea pig/Pig/Horse/Cow CD3e Antibody [Sodium Azide Free]

Spezifität: Cell surface and cytoplasmic

Aufreinigung: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Immunogen: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Anwendungsinformationen

Applikationshinweise: WB: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 min at RT
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: T-cell surface glycoprotein CD3 epsilon chain

Hintergrund:

Target Description: Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Gene Symbol: CD3E///T3E

Gen-ID: 916

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway