Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

DDB1 Antikörper (C-Term)

This anti-DDB1 antibody (ABIN6266199) is a Rabbit Polyclonal antibody detecting DDB1 in WB, IHC, ELISA, IF, ICC. Suitable for Human, Mouse, Rat.
Produktnummer ABIN6266199

Quick Overview for DDB1 Antikörper (C-Term) (ABIN6266199)

Target

Alle DDB1 Antikörper anzeigen
DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Reaktivität

  • 46
  • 24
  • 20
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 37
  • 8
  • 1
Kaninchen

Klonalität

  • 35
  • 11
Polyklonal

Konjugat

  • 35
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser DDB1 Antikörper ist unkonjugiert

Applikation

  • 31
  • 15
  • 14
  • 14
  • 11
  • 10
  • 8
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Spezifität

    DDB1 Antibody detects endogenous levels of total DDB1.

    Homologie

    Pig,Bovine,Horse,Rabbit,Chicken,Xenopus

    Aufreinigung

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    Immunogen

    A synthesized peptide derived from human DDB1, corresponding to a region within C-terminal amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store at -20 °C. Stable for 12 months from date of receipt.

    Haltbarkeit

    12 months
  • Target

    DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

    Andere Bezeichnung

    DDB1

    Hintergrund

    Description: Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.

    Gene: DDB1

    Molekulargewicht

    127kDa

    Gen-ID

    1642

    UniProt

    Q16531

    Pathways

    DNA Reparatur
Sie sind hier:
Chat with us!