EIF3I Antikörper (C-Term)

Produktnummer ABIN6261483

Der Kaninchen Polyklonal Anti-EIF3I-Antikörper wurde für WB und IHC validiert. Er ist geeignet, EIF3I in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für EIF3I Antikörper (C-Term) (ABIN6261483)

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF3I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-EIF3I Antikörper

Bindungsspezifität: C-Term

Spezifität: EIF3I Antibody detects endogenous levels of total EIF3I.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EIF3I, corresponding to a region within C-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu EIF3I

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Andere Bezeichnung: EIF3I

Hintergrund:

Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Gene: EIF3I

Molekulargewicht: 36 kDa

Gen-ID: 8668

UniProt: Q13347

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Ribonucleoprotein Complex Subunit Organization, Synthesis of DNA