FZD8 Antikörper (N-Term)

Produktnummer ABIN6258793

Dieses Anti-FZD8-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von FZD8 in WB, ELISA, IF und ICC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für FZD8 Antikörper (N-Term) (ABIN6258793)

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FZD8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-FZD8 Antikörper

Bindungsspezifität: N-Term

Spezifität: FZD8 Antibody detects endogenous levels of total FZD8.

Homologie: Pig,Bovine,Dog,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human FZD8, corresponding to a region within N-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu FZD8

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Andere Bezeichnung: FZD8

Hintergrund:

Description: Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.

Gene: FZD8

Molekulargewicht: 70 kDa

Gen-ID: 8325

UniProt: Q9H461

Pathways: WNT Signalweg