EZH1 Antikörper (Internal Region)

Produktnummer ABIN6258153

Der Kaninchen Polyklonal Anti-EZH1-Antikörper wurde für WB, ELISA, IHC, IF und ICC validiert. Er ist geeignet, EZH1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für EZH1 Antikörper (Internal Region) (ABIN6258153)

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EZH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-EZH1 Antikörper

Bindungsspezifität: Internal Region

Spezifität: EZH1 Antibody detects endogenous levels of total EZH1.

Homologie: Pig,Bovine,Horse,Sheep,Dog

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EZH1, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu EZH1

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Andere Bezeichnung: EZH1

Hintergrund:

Description: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

Gene: EZH1

Molekulargewicht: 85 kDa

Gen-ID: 2145

UniProt: Q92800