EHMT1 Antikörper (Internal Region)

Produktnummer ABIN6258149

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch EHMT1 in WB, ELISA, IF und ICC. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für EHMT1 Antikörper (Internal Region) (ABIN6258149)

Target: EHMT1 (Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EHMT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-EHMT1 Antikörper

Bindungsspezifität: Internal Region

Spezifität: EHMT1 Antibody detects endogenous levels of total EHMT1.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EHMT1, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Haltbarkeit: 12 months

Details zu EHMT1

Target: EHMT1 (Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1))

Andere Bezeichnung: EHMT1

Hintergrund:

Description: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.

Gene: EHMT1

Molekulargewicht: 170 kDa

Gen-ID: 79813

UniProt: Q9H9B1