EHMT1 Antikörper (Internal Region)

Produktnummer ABIN6258149

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EHMT1 in WB, ELISA, IF und ICC. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für EHMT1 Antikörper (Internal Region) (ABIN6258149)

Target: EHMT1 (Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EHMT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-EHMT1 Antikörper

Bindungsspezifität: Internal Region

Spezifität: EHMT1 Antibody detects endogenous levels of total EHMT1.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EHMT1, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Haltbarkeit: 12 months

Details zu EHMT1

Target: EHMT1 (Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1))

Andere Bezeichnung: EHMT1

Hintergrund:

Description: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.

Gene: EHMT1

Molekulargewicht: 170 kDa

Gen-ID: 79813

UniProt: Q9H9B1