POFUT1 Antikörper (C-Term)

Produktnummer ABIN6257766

Der Kaninchen Polyklonal Anti-POFUT1-Antikörper wurde für WB, IHC, ELISA, IF und ICC validiert. Er ist geeignet, POFUT1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für POFUT1 Antikörper (C-Term) (ABIN6257766)

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POFUT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-POFUT1 Antikörper

Bindungsspezifität: C-Term

Spezifität: POFUT1 Antibody detects endogenous levels of total POFUT1.

Homologie: Pig,Bovine,Horse,Sheep,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POFUT1, corresponding to a region within C-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu POFUT1

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Andere Bezeichnung: POFUT1

Hintergrund:

Description: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).

Gene: POFUT1

Molekulargewicht: 44 kDa

Gen-ID: 23509

UniProt: Q9H488

Pathways: Notch Signalweg, SARS-CoV-2 Protein Interaktom