SKP2 Antikörper (pSer64)

Produktnummer ABIN6255441

Der Kaninchen Polyklonal anti-SKP2 Antikörper wird verwendet zum Nachweis von SKP2 in Proben von Human und Maus. Er wurde validiert für WB und IHC.

Kurzübersicht für SKP2 Antikörper (pSer64) (ABIN6255441)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SKP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-SKP2 Antikörper

Bindungsspezifität: pSer64

Spezifität: Phospho-Skp2 (Ser64) Antibody detects endogenous levels of Skp2.

Kreuzreaktivität: Human, Maus

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Skp2 around the phosphorylation site of Ser64.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C.Stable for 12 months from date of receipt

Haltbarkeit: 12 months

Details zu SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Andere Bezeichnung: Skp2

Hintergrund:

Description: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

Gene: SKP2

Molekulargewicht: 48kDa

Gen-ID: 6502

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases