CEA Antikörper

Produktnummer ABIN6252839

Dieses Anti-CEA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CEA in WB, IHC, FACS, ELISA und IF. Geeignet für Human.

Kurzübersicht für CEA Antikörper (ABIN6252839)

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CEA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Klon: C66-1009

Produktdetails anti-CEA Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Human full-length recombinant CEA protein was used as the immunogen for this antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: ELISA: order BSA-free format (3)

Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. Clone C66/1009 (recommended detect) will pair with clones < a href=../tds/cea-antibody-c661030-v2375>C66/1030 and < a href=../tds/cea-antibody-c66261-v2088>C66/261 (recommended capture) by ELISA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Aliquot and Store at 2-8C. Avoid freez-thaw cycles.

Details zu CEA

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Andere Bezeichnung: CEA

Hintergrund: This antibody recognizes proteins of 80-200  kDa, identified as different members of the CEA (Carcinoembryonic Antigen) family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. The antibody shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). CEA antibody positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Gen-ID: 1048

UniProt: P06731