TNFSF15 Antikörper

Produktnummer ABIN6252817

Der Maus Monoklonal Anti-TNFSF15-Antikörper wurde für WB, IHC, IF und FACS validiert. Er ist geeignet, TNFSF15 in Proben von Human zu detektieren.

Kurzübersicht für TNFSF15 Antikörper (ABIN6252817)

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNFSF15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: TLRM1-1

Produktdetails anti-TNFSF15 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: A human partial recombinant protein was used as the immunogen for this TL1A antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

Western blot: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TL1A antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9, for 10-20 min followed by cooling at RT for 20 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu TNFSF15

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Andere Bezeichnung: TL1A

Hintergrund: TNF ligand-related molecule 1 is an anti-angiogenic cytokine that is also called Tumor necrosis factor superfamily, member 15 (TNFSF15) and Vascular endothelial growth inhibitor (VEGI/VEGI192A). This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Reduced expression of TL1A has been reported as a marker of poor prognosis in breast cancer.

Gen-ID: 9966

UniProt: O95150

Pathways: Positive Regulation of Endopeptidase Activity, Autophagie