PAX7 Antikörper (AA 300-600)

Produktnummer ABIN6252801

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PAX7 in IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PAX7 Antikörper (AA 300-600) (ABIN6252801)

Target: PAX7 (Paired Box 7 (PAX7))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAX7 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: PBPX7

Produktdetails anti-PAX7 Antikörper

Bindungsspezifität: AA 300-600

Aufreinigung: Protein G affinity chromatography

Immunogen: A recombinant fragment (aa300-600) was used as the immunogen for the PAX7 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the PAX7 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris buffer with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Andere Bezeichnung: PAX7

Hintergrund: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

Gen-ID: 5081

UniProt: P23759