ENO2/NSE Antikörper (AA 416-433)

Produktnummer ABIN6252647

Dieses Anti-ENO2/NSE-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ENO2/NSE in WB, IHC, IF und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für ENO2/NSE Antikörper (AA 416-433) (ABIN6252647)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ENO2/NSE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: ENO2-1375

Produktdetails anti-ENO2/NSE Antikörper

Bindungsspezifität: AA 416-433

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot: 0.5-1 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

IF: 1-2 μg/mL

IHC (FFPE): 0.1-0.2 μg/mL for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Andere Bezeichnung: Neuron Specific Enolase

Hintergrund: Recognizes a protein of about 50  kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Gen-ID: 2026

UniProt: P09104