CD45RO Antikörper

Produktnummer ABIN6252434

Der Maus Monoklonal anti-CD45RO Antikörper (Klon SPM125) (ABIN6252434) detektiert spezifisch CD45RO in FACS, IF und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Schimpanse und Marmoset.

Kurzübersicht für CD45RO Antikörper (ABIN6252434)

Target: CD45RO

Reaktivität: Human, Schimpanse, Marmoset

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD45RO Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: SPM125

Produktdetails anti-CD45RO Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as immunogen for this anti-CD45RO antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells

Immunofluorescence: 0.5-1 μg/mL

Immunohistology (FFPE): 0.25-0.5 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Due to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu CD45RO

Target: CD45RO

Andere Bezeichnung: CD45RO

Hintergrund: Recognizes a 180-185  kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Gen-ID: 5788

UniProt: P08575