CD3 epsilon Antikörper (Cytoplasmic Domain)

Produktnummer ABIN6252415

Der Maus Monoklonal anti-CD3 epsilon Antikörper wird verwendet zum Nachweis von CD3 epsilon in Proben von Human, Maus, Ratte, Schwein, Rind (Kuh), Pferd, Kaninchen und Meerschweinchen. Er wurde validiert für FACS, WB, IF und IHC (p).

Kurzübersicht für CD3 epsilon Antikörper (Cytoplasmic Domain) (ABIN6252415)

Target: CD3 epsilon (CD3E)

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: PC3-188A

Produktdetails anti-CD3 epsilon Antikörper

Bindungsspezifität: AA 156-168, Cytoplasmic Domain

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Optimal dilution of the CD3e antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3e

Hintergrund: Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Gen-ID: 916

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway