CD3 epsilon Antikörper

Produktnummer ABIN6252412

Der Kaninchen Polyklonal anti-CD3 epsilon Antikörper wird verwendet zum Nachweis von CD3 epsilon in Proben von Human, Maus, Ratte, Schwein, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen, Huhn, Hund, Schaf, Hamster, Cat, Cryptomys amatus (Mole rat), Elephant, Frosch, Opossum und Primate. Er wurde validiert für FACS, WB und IP.

Kurzübersicht für CD3 epsilon Antikörper (ABIN6252412)

Target: CD3 epsilon (CD3E)

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen, Huhn, Hund, Schaf, Hamster, Cat, Cryptomys amatus (Mole rat), Elephant, Frosch, Opossum, Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-CD3 epsilon Antikörper

Aufreinigung: Protein A purified CD3e antibody

Immunogen: Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: use the CD3e antibody at 0.5-1 μg/million cells

WB: 0.5-1.0 μg/mL

IP: 1-2 μg/500 μg protein lysate



The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3e

Hintergrund: This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.

Gen-ID: 916

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway