ICAM-3/CD50 Antikörper
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- Target Alle ICAM-3/CD50 (ICAM3) Antikörper anzeigen
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser ICAM-3/CD50 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.
- Klon
- 101-1D2
- Isotyp
- IgG2a kappa
- Top Product
- Discover our top product ICAM3 Primärantikörper
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- Applikationshinweise
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Optimal dilution of the ICAM-3 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- Andere Bezeichnung
- ICAM-3 (ICAM3 Produkte)
- Synonyme
- CD50 antikoerper, CDW50 antikoerper, ICAM-R antikoerper, ICAM-3 antikoerper, ICAM3 antikoerper, intercellular adhesion molecule 3 antikoerper, ICAM3 antikoerper, Icam3 antikoerper
- Hintergrund
- Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This mAb is excellent for staining of formalin/paraffin tissues.
- Gen-ID
- 3385
- UniProt
- P32942
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