Nucleolin Antikörper

Produktnummer ABIN6252098

Dieses Anti-Nucleolin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Nucleolin in WB, IF, FACS und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für Nucleolin Antikörper (ABIN6252098)

Target: Nucleolin (NCL)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nucleolin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: NPC23-2

Produktdetails anti-Nucleolin Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: IF: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Western blot: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the Nucleolin antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Nucleolin

Target: Nucleolin (NCL)

Andere Bezeichnung: Nucleolin

Hintergrund: Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Gen-ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization