MFGE8 Antikörper

Produktnummer ABIN6252079

Der Maus Monoklonal Anti-MFGE8-Antikörper wurde für WB, FACS, IF und IHC (p) validiert. Er ist geeignet, MFGE8 in Proben von Human und Hund zu detektieren.

Kurzübersicht für MFGE8 Antikörper (ABIN6252079)

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MFGE8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MFG-06

Produktdetails anti-MFGE8 Antikörper

Spezifität: Does not react with mouse and rat

Aufreinigung: Protein G affinity chromatography

Immunogen: A human milk fat globule membrane preparation was used as the immunogen for the MFGE8 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the MFGE8 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Andere Bezeichnung: MFGE8

Hintergrund: Recognizes a protein of 40-45  kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin, or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Gen-ID: 4240

UniProt: Q08431

Pathways: SARS-CoV-2 Protein Interaktom