TTF1 Antikörper

Produktnummer ABIN6252067

Der Maus Monoklonal Anti-TTF1-Antikörper wurde für FACS, IHC (p) und IF validiert. Er ist geeignet, TTF1 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für TTF1 Antikörper (ABIN6252067)

Target: TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TTF1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Klon: HBNK2-1

Produktdetails anti-TTF1 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant protein was used as the immunogen for the TTF1 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the TTF1 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu TTF1

Target: TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))

Andere Bezeichnung: TTF1

Hintergrund: Thyroid transcription factor-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. TTF-1 reactivity is also seen in thyroid malignancies.

Gen-ID: 7080

UniProt: P43699

Pathways: Thyroid Hormone Synthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour