Involucrin Antikörper (AA 421-568)

Produktnummer ABIN6252058

Der Maus Monoklonal Anti-Involucrin-Antikörper wurde für IHC, IF und FACS validiert. Er ist geeignet, Involucrin in Proben von Human, Hund, Schwein, Gorilla und Owl Monkey zu detektieren.

Kurzübersicht für Involucrin Antikörper (AA 421-568) (ABIN6252058)

Target: Involucrin (IVL)

Reaktivität: Human, Hund, Schwein, Gorilla, Owl Monkey

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Involucrin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: SPM259

Produktdetails anti-Involucrin Antikörper

Bindungsspezifität: AA 421-568

Spezifität: Does not react with Mouse

Aufreinigung: Protein G affinity chromatography

Immunogen: Purified involucrin from human keratinocytes was used as the immunogen for this Involucrin antibody. Its epitope maps between amino acids 421-568 of human involucrin.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The optimal dilution of the Involucrin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37oC. Enzyme digestion is better than citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Involucrin

Target: Involucrin (IVL)

Andere Bezeichnung: Involucrin

Hintergrund: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Gen-ID: 3713

UniProt: P07476