POMC Antikörper (N-Term)

Produktnummer ABIN6251838

Dieses Maus Monoklonal-Antikörper erkennt spezifisch POMC in WB, IHC, FACS und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für POMC Antikörper (N-Term) (ABIN6251838)

Target: POMC (Proopiomelanocortin (POMC))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser POMC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 57

Produktdetails anti-POMC Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Protein G affinity chromatography

Immunogen: An N-terminal peptide from the ACTH region of human POMC was used as the immunogen for this POMC antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

The optimal dilution of the ACTH antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu POMC

Target: POMC (Proopiomelanocortin (POMC))

Andere Bezeichnung: POMC

Hintergrund: POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin.

Gen-ID: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour