ALK Antikörper (C-Term)

Produktnummer ABIN6243474

Der Kaninchen Polyklonal Anti-ALK-Antikörper wurde für WB validiert. Er ist geeignet, ALK in Proben von Human zu detektieren.

Kurzübersicht für ALK Antikörper (C-Term) (ABIN6243474)

Target: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ALK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB50500

Produktdetails anti-ALK Antikörper

Bindungsspezifität: AA 1466-1500, C-Term

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ALK antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1466-1500 amino acids from the C-terminal region of human ALK.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Details zu ALK

Target: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Andere Bezeichnung: ALK

Hintergrund: Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.

Molekulargewicht: 176442

UniProt: Q9UM73

Pathways: RTK Signalweg