DMPO Antikörper

Produktnummer ABIN619793

Der Maus Monoklonal anti-DMPO Antikörper wird verwendet zum Nachweis von DMPO. Er wurde validiert für IP, ELISA, WB, ICC und IST.

Kurzübersicht für DMPO Antikörper (ABIN619793)

Target: DMPO

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunoprecipitation (IP), ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immuno-Spin Trapping (IST)

Klon: N1664A

Produktdetails

Verwendungszweck: Anti-DMPO | DMPO nitrone adduct (clone N1664A)

Kreuzreaktivität (Details): DMPO (species independent)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: DMPO (species independent)

Aufreinigung: Protein G purified

Immunogen: 5

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 1-10 μg/mL (ELISA), 10 μg/mL (ICC), 25 μg (IP), 1-10 μg/mL (WB)

Kommentare:

Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Total IgG. Protein G purified.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for one year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Haltbarkeit: 12 months

Antigendetails

Target: DMPO

Substanzklasse: Chemical

Hintergrund:

Background: DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.

Additional information: Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.

Molekulargewicht: 90 kDa