DMPO Antikörper (PE)

Produktnummer ABIN2482182

Produktdetails

Target: DMPO

Wirt: Maus

Klonalität: Monoklonal

Konjugat: PE

Applikation: Immunoprecipitation (IP), ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Antibody Array (AA)

Spezifität: Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.

Aufreinigung: Protein G Purified

Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin

Klon: N1664A

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• ELISA (1:100)
• IP (25 μg)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN2482182 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Antigendetails

Target: DMPO

Substanzklasse: Chemical

Hintergrund: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7).