TNFSF15 Antikörper (CF®640R)

Produktnummer ABIN6189578

Der CF®640R-konjugierte Maus Monoklonal anti-TNFSF15 Antikörper (Klon VEGI-1283) (ABIN6189578) detektiert spezifisch TNFSF15 in WB, IHC, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TNFSF15 Antikörper (CF®640R) (ABIN6189578)

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNFSF15 Antikörper ist konjugiert mit CF®640R

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: VEGI-1283

Produktdetails anti-TNFSF15 Antikörper (CF®640R)

Verwendungszweck: Mouse Monoclonal anti-VEGI (VEGI/1283), CF640R Conjugate

Produktmerkmale: VEGI is an anti-angiogenic cytokine that belongs to tumor necrosis factor superfamily, member 15 (TNFSF15). This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Reduced expression of VEGI has been reported as a marker of poor prognosis in breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length VEGI fragment

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 1-2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Western blotting 1-2 μg/mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Endothelial cells. Colon, intestine, placenta, lung, liver, kidney, pancreas, spleen and prostate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu TNFSF15

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Andere Bezeichnung: VEGI

Hintergrund: TNF lig,-related molecule 1 (TL1A), Tumor necrosis factor (lig,) superfamily member 15 (TNFSF15), Vascular endothelial growth inhibitor 192a, Vascular endothelial growth inhibitor (VEGI), VEGI192A

Molekulargewicht: 22 kDa

Gen-ID: 9966, 241382

UniProt: O95150

Pathways: Positive Regulation of Endopeptidase Activity, Autophagie