MYOD1 Antikörper

Produktnummer ABIN6188471

Dieses Anti-MYOD1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MYOD1 in IF, FACS, IHC (f) und Diff. Geeignet für Human, Maus, Ratte und Huhn.

Kurzübersicht für MYOD1 Antikörper (ABIN6188471)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Differentiation (Diff)

Klon: 5-8A

Produktdetails anti-MYOD1 Antikörper

Verwendungszweck: Mouse Monoclonal anti-MyoD1 (5.8A), Purified, BSA-free

Produktmerkmale: This antibody recognizes a phosphor-protein of 45 kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Recombinant mouse MyoD1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Rhabdomyosarcoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MyoD1

Hintergrund: BHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Molekulargewicht: 45 kDa

Gen-ID: 4654, 181768

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development