Villin 1 Antikörper (AA 179-311) (Biotin)

Produktnummer ABIN6186943

Der Maus Monoklonal Anti-Villin 1-Antikörper wurde für IHC, WB, IF und FACS validiert. Er ist geeignet, Villin 1 in Proben von Human zu detektieren.

Kurzübersicht für Villin 1 Antikörper (AA 179-311) (Biotin) (ABIN6186943)

Target: Villin 1 (VIL1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Villin 1 Antikörper ist konjugiert mit Biotin

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: VIL1-1314

Produktdetails anti-Villin 1 Antikörper (Biotin)

Bindungsspezifität: AA 179-311

Verwendungszweck: Mouse Monoclonal anti-Villin (VIL1/1314), Biotin Conjugate

Produktmerkmale: This antibody recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Villin fragment of 133 amino acid residues (aa179-311) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 0.25-0.5 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Western blotting 1-2 μg/mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

A549, HepG2 and HCT116 cells. Colon or Rectum.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Villin 1

Target: Villin 1 (VIL1)

Andere Bezeichnung: Villin

Hintergrund: VIL1, Villin-1, Villin1

Molekulargewicht: 93 kDa

Gen-ID: 7429, 654595

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization