CDC20 Antikörper (CF®594)

Produktnummer ABIN6185494

Der Maus Monoklonal Anti-CDC20-Antikörper wurde für FACS, IF und IHC (f) validiert. Er ist geeignet, CDC20 in Proben von Human zu detektieren.

Kurzübersicht für CDC20 Antikörper (CF®594) (ABIN6185494)

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDC20 Antikörper ist konjugiert mit CF®594

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: AR12

Produktdetails anti-CDC20 Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-Cdc20 (AR12), CF594 Conjugate

Produktmerkmale: Cyclins, regulatory subunits that associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Urea-denatured His6 Cdc20 human recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Ramos or HeLa cells. Tonsil or gastric carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CDC20

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Andere Bezeichnung: Cdc20

Hintergrund: CDC20, CDC20A, p55CDC, P55CDC-LSB

Molekulargewicht: 55 kDa

Gen-ID: 991, 524947

UniProt: Q12834