CD70 Antikörper (CF®405S)

Produktnummer ABIN6185090

Dieses Anti-CD70-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD70 in ELISA, FACS, IP, IF, IHC (fro), Coat und ProA. Geeignet für Human.

Kurzübersicht für CD70 Antikörper (CF®405S) (ABIN6185090)

Target: CD70 (CD70 Molecule (CD70))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD70 Antikörper ist konjugiert mit CF®405S

Applikation: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Coating (Coat), Proliferation Assay (ProA)

Klon: BU69

Produktdetails anti-CD70 Antikörper (CF®405S)

Verwendungszweck: Mouse Monoclonal anti-CD70 (BU69), CF405S Conjugate

Produktmerkmale: This antibody recognizes a protein of 30 kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This MAb blocks the interaction between CD27 and CD70, and has been shown toinhibit T cell proliferation induced by dendritic cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human WM-1 (Waldenstrom s macroglobulinemia) cell line

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (frozen) 0.5-1 μg/mL

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• ELISA For coating, order Ab without BSA
• Inhibits T cell proliferation induced by dendritic cells
• Optimal dilution for a specific application should be determined by user

Kommentare:

Raji cells. Lymph node or tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CD70

Target: CD70 (CD70 Molecule (CD70))

Andere Bezeichnung: CD70

Hintergrund: CD27 ligand (CD27L), CD27LG, Ki24, Surface antigen CD70, Tumor necrosis factor (ligand) superfamily, member 7 (TNFSF7)

Molekulargewicht: ~30 kDa

Gen-ID: 970

UniProt: P32970

Pathways: Cancer Immune Checkpoints