CD5 Antikörper

Produktnummer ABIN6184973

Der Maus Monoklonal anti-CD5 Antikörper (Klon C5-473 CD5-54-F6) (ABIN6184973) detektiert spezifisch CD5 in FACS, IF und IHC (f). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Rind (Kuh) und Kaninchen.

Kurzübersicht für CD5 Antikörper (ABIN6184973)

Target: CD5

Reaktivität: Human, Ratte, Rind (Kuh), Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: C5-473 CD5-54-F6

Produktdetails anti-CD5 Antikörper

Verwendungszweck: Mouse Monoclonal anti-CD5 (C5/473 + CD5/54/F6), Purified, with BSA

Produktmerkmale: This antibody recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma ( ) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Human CD5 recombinant protein (C5/473), A synthetic peptide from the intracellular region of human CD5 (CD5/54/F6)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

293T, Ramos, or MOLT-4 Cells. Tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu CD5

Target: CD5

Andere Bezeichnung: CD5

Hintergrund: CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5

Molekulargewicht: 67 kDa

Gen-ID: 921, 58685

UniProt: P06127