CD235a/GYPA Antikörper (CF®555)

Produktnummer ABIN6184720

Der Maus Monoklonal Anti-CD235a/GYPA-Antikörper wurde für FACS, IHC und IF validiert. Er ist geeignet, CD235a/GYPA in Proben von Human zu detektieren.

Kurzübersicht für CD235a/GYPA Antikörper (CF®555) (ABIN6184720)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD235a/GYPA Antikörper ist konjugiert mit CF®555

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: JC159

Produktdetails anti-CD235a/GYPA Antikörper (CF®555)

Verwendungszweck: Mouse Monoclonal anti-Glycophorin-A / CD235a (JC159), CF555 Conjugate

Produktmerkmale: This antibody recognizes a sialoglycoprotein of 39 kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Formalin fixed membrane from a Hairy cell leukemia

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Erythrocytes in any human tissues

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Andere Bezeichnung: Glycophorin-A / CD235a

Hintergrund: Blood group--MN locus, GPA, GPErik, GpMiIII, GPSAT, GYPA, MN sialoglycoprotein, MNS, PAS2, Sialoglycoprotein alpha

Molekulargewicht: 39 kDa

Gen-ID: 2993, 2994

UniProt: P02724

Pathways: Maintenance of Protein Location