Rekombinanter CD15 Antikörper (CF®647)

Produktnummer ABIN6183844

Der Kaninchen Monoklonal Anti-CD15-Antikörper wurde für FACS, IHC und IF validiert. Er ist geeignet, CD15 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Rekombinanter CD15 Antikörper (CF®647) (ABIN6183844)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD15 Antikörper ist konjugiert mit CF®647

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: FUT4-1478R

Produktdetails anti-CD15 Antikörper (CF®647)

Verwendungszweck: Recombinant Rabbit Monoclonal anti-CD15 / FUT4 (FUT4/1478R), CF647 Conjugate

Produktmerkmale: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95 % of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human neutrophils

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Flow cytometry 2-4 μg/million cells
• Immunofluorescence 2-4 μg/mL
• Immunohistology (formalin) 5-10 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

U937 cells, Reed-Sternberg',s cells in Hodgkin',s lymphoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Andere Bezeichnung: CD15 / FUT4

Molekulargewicht: 220 kDa

Gen-ID: 2526, 654379

UniProt: P22083