Rekombinanter Nkx2-2 Antikörper (CF®405S)

Produktnummer ABIN6181556

Dieses Anti-Nkx2-2-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von Nkx2-2 in IF, IHC und FACS. Geeignet für Human.

Kurzübersicht für Rekombinanter Nkx2-2 Antikörper (CF®405S) (ABIN6181556)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Nkx2-2 Antikörper ist konjugiert mit CF®405S

Applikation: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon: NX2-1422R

Produktdetails anti-Nkx2-2 Antikörper (CF®405S)

Verwendungszweck: Recombinant Rabbit Monoclonal anti-NKX2.2 (NX2/1422R), CF405S Conjugate

Produktmerkmale: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human NKX2.2 protein fragment (exact sequence is proprietary)

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Pancreas or Ewing s sarcoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Andere Bezeichnung: NKX2.2

Hintergrund: Homeobox protein NK-2 homolog B, NK2 transcription factor like protein B, NK2 transcription factor related locus 2, NKX22, Nkx2b, tinman

Molekulargewicht: 40-50 kDa

Gen-ID: 4821, 516922

UniProt: O95096

Pathways: Dopaminergic Neurogenesis