SOX10 Antikörper (AA 115-269) (CF®568)

Produktnummer ABIN6181114

Der Maus Monoklonal Anti-SOX10-Antikörper wurde für WB, FACS und IF validiert. Er ist geeignet, SOX10 in Proben von Human zu detektieren.

Kurzübersicht für SOX10 Antikörper (AA 115-269) (CF®568) (ABIN6181114)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOX10 Antikörper ist konjugiert mit CF®568

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: SOX10-992

Produktdetails anti-SOX10 Antikörper (CF®568)

Bindungsspezifität: AA 115-269

Verwendungszweck: Mouse Monoclonal anti-SOX10 (SOX10/992), CF568 Conjugate

Produktmerkmale: This antibody recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (aa 115-269) of human SOX10 protein with hexa-histidine tag

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HepG2 cells. Melanomas, breast carcinomas, gliomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Andere Bezeichnung: SOX10

Hintergrund: DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E (WS2E), WS4, Waardenburg syndrome type 4C (WS4C)

Molekulargewicht: 49-58 kDa

Gen-ID: 6663, 376984

UniProt: P56693

Pathways: Chromatin Binding