MITF Antikörper (CF®594)

Produktnummer ABIN6179908

Der Maus Monoklonal Anti-MITF-Antikörper wurde für IHC, IF und FACS validiert. Er ist geeignet, MITF in Proben von Human zu detektieren.

Kurzübersicht für MITF Antikörper (CF®594) (ABIN6179908)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MITF Antikörper ist konjugiert mit CF®594

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: D5 MITF-915

Produktdetails anti-MITF Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-MiTF (Microphthalmia Transcription Factor) (D5 + MITF/915), CF594 Conjugate

Produktmerkmale: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: NH2 terminus fragment of human Mi protein (D5), Recombinant human MiTF protein (MITF/915)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Does not react with mouse or rat, others not tested

• Immunohistology (formalin)
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Jurkat, A-431, HeLa or 501 Mel human melanoma cells or Melanoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Andere Bezeichnung: MiTF

Hintergrund: BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A

Molekulargewicht: 52-56 kDa (doublet)

Gen-ID: 4286

UniProt: O75030

Pathways: Chromatin Binding